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Cronología cerámica en Cancuén: historia de una ciudad del Clásico Tardío
Forné, Mélanie;Alvarado, Silvia;Torres, Paola;
Estudios de cultura maya , 2011,
Abstract: cancuén archaeological site is relevant within the late classic maya context because of its geographical position on pasión river, between lowlands and highlands of guatemala, from where it controlled one of the greatest trade route between both areas. this has been evidenced by the excavation of a workshop where jade artifacts where produced, which is a unique discovery in maya lowlands. the analysis of ceramics in cancuén represents a double challenge: first, it may define cancuén's culturally, highlighting a mix of ceramic influences coming from different neighboring regions. a rapid presentation of the typology shows a quite complex panorama for exchange and local production of vessels. the second challenge is about establishing a fine chronology for the only 150 years of occupation. by confronting ceramic data with epigraphy and local political events, it has been possible to establish a detailed and complete chronology, one of the first in this frontier zone, between lowlands and highlands.
Presentación
Silvia Borelli,Sara Victoria Alvarado,Pablo Vommaro
Utopia y Praxis Latinoamericana , 2012,
Abstract:
Cronología cerámica en Cancuén: historia de una ciudad del Clásico Tardío Ceramic Chronology at Cancuén: a History of a Late Classic Period City
Mélanie Forné,Silvia Alvarado,Paola Torres
Estudios de cultura maya , 2011,
Abstract: El sitio arqueológico Cancuén se destaca en el área maya del Clásico Tardío por su localización en el río de la Pasión, entre las Tierras Altas y las Tierras Bajas de Guatemala; desde allí la ciudad controló parte del flujo de bienes entre ambas áreas. Esto pudo comprobarse a través de la excavación de un taller de manufactura de artefactos de jade, cuya existencia fue un hecho único en las ciudades de Tierras Bajas. El estudio de la cerámica de este sitio presenta un doble reto: primero, ubicar Cancuén culturalmente, revelando una mezcla de influencias cerámicas procedentes de varias regiones vecinas. Una presentación sintética de la tipología permite vislumbrar la complejidad de las redes de intercambio y producción local de las vasijas. El segundo reto es el de establecer una cronología fina para los apenas 150 a os de duración de la ocupación del sitio. La confrontación de la tipología cerámica con los datos epigráficos y los eventos políticos locales, permite establecer una secuencia cronológica completa, una de las primeras para esta zona fronteriza entre Tierras Bajas y Tierras Altas. Cancuén archaeological site is relevant within the Late Classic Maya context because of its geographical position on Pasión River, between Lowlands and Highlands of Guatemala, from where it controlled one of the greatest trade route between both areas. This has been evidenced by the excavation of a workshop where jade artifacts where produced, which is a unique discovery in Maya Lowlands. The analysis of ceramics in Cancuén represents a double challenge: first, it may define Cancuén's culturally, highlighting a mix of ceramic influences coming from different neighboring regions. A rapid presentation of the typology shows a quite complex panorama for exchange and local production of vessels. The second challenge is about establishing a fine chronology for the only 150 years of occupation. By confronting ceramic data with epigraphy and local political events, it has been possible to establish a detailed and complete chronology, one of the first in this frontier zone, between Lowlands and Highlands.
Regional Development Model Based on Organic Production of Nopal  [PDF]
Tavera Cortes María Elena, Escamilla García Pablo Emilio, Alvarado Raya Horacio, Salinas Callejas Edmar, Galicia Villanueva Silvia
Modern Economy (ME) , 2014, DOI: 10.4236/me.2014.53025
Abstract:

The compost production has been poorly explored in the agricultural areas of Mexico City. Farmers tend to use fresh manure as fertilizer on soil cultivation. The borough of Milpa Alta, as the main nopal producer in Mexico, requires a high amount of fertilizers to guarantee the fulfillment of yields and times of cultivation. Nevertheless the wide use of manure as only fertilizer implies several negative aspects not only for the crop but also for the environment. The compost is presented as a feasible option to improve the quality of crops and also to diminish the production cost. Compost has features and benefits that are directly reflected in the decrease of costs against the economic and conservation costs emerged with the use of fresh manure. The present research aims to evaluate the alternative use of harmless compost with quality to be used in agriculture. It was carried out through an analysis in an experimental plant built exclusively for the project. The main goal is to demonstrate the cost reduction in the nopal production in Milpa Alta by using compost instead of fresh manure.

Torture in Honduras: Therapeutic Experiences of 2007-2008 and the Aftermath of the Coup  [PDF]
Arely Alvarado
Open Journal of Therapy and Rehabilitation (OJTR) , 2014, DOI: 10.4236/ojtr.2014.22011
Abstract: Background: The Center for Prevention, Treatment and Rehabilitation for Torture Victims and their Family (CPTRT) participated in the “Professionalization facilitated through training in key healthcare services for torture victims” project coordinated by the International Rehabilitation Council for Torture Victims (IRCT). Centers like CPTRT are typically created and run by small groups of healthcare professionals and human rights activists, often at great personal cost and risk [1]. The purpose of the project was to document the physical and psychological damage caused by torture as well as the results of the medical and psychological therapy. It also addresses: the socio-demographic profile of the victims, methods of torture and/or cruel, inhuman and degrading (CID), treatment, causal factors, and treatment effect. Material and Method: Psychometric and non-psychometric techniques were applied in order to obtain a baseline of the victims’ state of mental health, socio-demographic background, clinical and torture exposure, as well as evaluating the impact of the medical-psychological therapy at three and six months, respectively. The sample of torture victims is presented by the use of descriptive statistics and the effects during the study are presented with repeated measures analysis of variance. Results: The victims mostly experienced depression and anxiety and to a lesser extent, post-traumatic stress and somatizations as a response to the traumatic events they endured. The analysis demonstrates a significant drop in symptoms over the six months of the study in those victims who participated in the entire project. Conclusion: Although the several limitations and unforeseen situations arose in a study of this type, a holistic, multidisciplinary and systematic approach is able to decrease disorders moderately, but not without taking into account the sociopolitical contexts and the obstacles appearing therein, which should also be considered and studied through triangulated methodologies that provide a broader perspective for exploring a complex phenomenon like torture. Therefore, a standard rehabilitation program is neither possible nor desirable in the field of torture treatment (this is amplified in a political context of a coup).
The Phenols of the Genus Agave (Agavaceae)  [PDF]
Norma Almaraz-Abarca, Eli Amanda Delgado-Alvarado, José Antonio ávila-Reyes, José Natividad Uribe-Soto, Laura Silvia González-Valdez
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2013, DOI: 10.4236/jbnb.2013.43A002
Abstract:

A review is presented about the studies that have been carried out on the phenolic composition of the species of the genus Agave in order to show which species of the genus has been analyzed, what is the potential of the species of Agave as source of phenolics with important biological activities, and what is the significance of the phenolic profiles as chemotaxonomic markers in the genus. The results of the analysis of a dozen of Agave species reveal an important richness of phenolic compounds, including flavonoids, homoisoflavonoids, and phenolic acids, and a broad diversity of biological activities associated to those compounds, as antioxidant, antibacterial, antifungal, antinematod, and immunomodulatory capabilities. The phenols of Agave also have a major potential as specific chemotaxonomic markers.

Rotavirus A-specific single-domain antibodies produced in baculovirus-infected insect larvae are protective in vivo
Gómez-Sebastián Silvia,Nu?ez Maria C,Garaicoechea Lorena,Alvarado Carmen
BMC Biotechnology , 2012, DOI: 10.1186/1472-6750-12-59
Abstract: Background Single-domain antibodies (sdAbs), also known as nanobodies or VHHs, are characterized by high stability and solubility, thus maintaining the affinity and therapeutic value provided by conventional antibodies. Given these properties, VHHs offer a novel alternative to classical antibody approaches. To date, VHHs have been produced mainly in E. coli, yeast, plants and mammalian cells. To apply the single-domain antibodies as a preventive or therapeutic strategy to control rotavirus infections in developing countries (444,000 deaths in children under 5 years of age) has to be minimized their production costs. Results Here we describe the highly efficient expression of functional VHHs by the Improved Baculovirus Expression System (IBES technology), which uses a baculovirus expression vector in combination with Trichoplusia ni larvae as living biofactories. Two VHHs, named 3B2 and 2KD1, specific for the inner capsid protein VP6 of Group A rotavirus, were expressed in insect larvae. The IBES technology achieved very high expression of 3B2 and 2KD1, reaching 2.62% and 3.63% of the total soluble protein obtained from larvae, respectively. These expression levels represent up to 257 mg/L of protein extract after insect processing (1 L extract represents about 125 g of insect biomass or about 375 insect larvae). Larva-derived antibodies were fully functional when tested in vitro and in vivo, neutralizing Group A rotaviruses and protecting offspring mice against rotavirus-induced diarrhea. Conclusions Our results open up the possibility of using insects as living biofactories (IBES technology) for the cost-efficient production of these and other fully functional VHHs to be used for diagnostic or therapeutic purposes, thereby eliminating concerns regarding the use of bacterial or mammalian cells. To the best of our knowledge, this is the first time that insects have been used as living biofactories to produce a VHH molecule.
Flavonoids and Ellagitannins Characterization, Antioxidant and Cytotoxic Activities of Phyllanthus acuminatus Vahl
Diego Alvarado,Elizabeth Arnaez,Felipe Vargas,Gabriela Azofeifa,Ileana Moreira,Mirtha Navarro,Pei Chen,Silvia Quesada
- , 2017, DOI: 10.3390/plants6040062
Abstract: The phenolic composition of leaves from Phyllanthus acuminatus L., a plant commonly used in Costa Rica as traditional medicine, was studied using UPLC-ESI-MS on an enriched phenolic extract. A total of 20 phenolic compounds were identified, comprising eight flavonoids (two flavanones—pinocembrin isomers and six derivatives from apigenin, chrysin, quercetin, and kaempferol); seven ellagitannins, two flavan-3-ols (prodelphinidin B dimer and (epi)gallocatechin); and three phenolic acids (ellagic acid, trimethylellagic acid, and ferulic acid). All of these compounds are reported for the first time in P. acuminatus, while previously reported in the genus Phyllanthus. Antioxidant evaluation was performed for P. acuminatus phenolic extract obtaining DPPH results with a remarkably low IC50 value of 0.15 μg/mL. Also, cytotoxicity on gastric AGS and colon SW20 adenocarcinoma cell lines was evaluated, and highly promising results were obtained, with IC50 values of 11.3 μg/mL and 10.5 μg/mL, respectively. Furthermore, selectivity index values obtained when comparing cytotoxicity on normal Vero cells was SI > 20 for both cancer cell lines, indicating a particularly high selectivity. Additionally, Justicidin B, a metabolite extensively studied for its antitumoral activity, was isolated from a non-polar extract of P. acuminatus, and comparatively evaluated for both bioactivities. The DPPH value obtained for Justicidin B was moderate (IC50 = 14.28 μg/mL), while cytotoxicity values for both AGS (IC50 = 19.5 μg/mL) and SW620 (IC50 = 24.8 μg/mL) cell lines, as well as selectivity when compared with normal Vero cells (SI = 5.4 and 4.2 respectively), was good, but lower than P. acuminatus extract. These preliminary results suggest that P. acuminatus enriched phenolic extract containing flavonoids, ellagitannins, flavan-3-ols, and phenolic acids, reported for the first time in this plant, could be of interest for further cancer cytotoxicity studies to elucidate structure–bioactivity relationships, and the molecular mechanisms and pathways
Proanthocyanidin Characterization, Antioxidant and Cytotoxic Activities of Three Plants Commonly Used in Traditional Medicine in Costa Rica: Petiveria alliaceae L., Phyllanthus niruri L. and Senna reticulata Willd.
Diego Alvarado,Elizabeth Arnaez,Gabriela Azofeifa,Ileana Moreira,Maria J. Monagas,Mirtha Navarro,Silvia Quesada
- , 2017, DOI: 10.3390/plants6040050
Abstract: The phenolic composition of aerial parts from Petiveria alliaceae L., Phyllanthus niruri L. and Senna reticulata Willd., species commonly used in Costa Rica as traditional medicines, was studied using UPLC-ESI-TQ-MS on enriched-phenolic extracts. Comparatively, higher values of total phenolic content (TPC), as measured by the Folin-Ciocalteau method, were observed for P. niruri extracts (328.8 gallic acid equivalents/g) than for S. reticulata (79.30 gallic acid equivalents/g) whereas P. alliaceae extract showed the lowest value (13.45 gallic acid equivalents/g). A total of 20 phenolic acids and proanthocyanidins were identified in the extracts, including hydroxybenzoic acids (benzoic, 4-hydroxybenzoic, gallic, prochatechuic, salicylic, syringic and vanillic acids); hydroxycinnamic acids (caffeic, ferulic, and p-coumaric acids); and flavan-3-ols monomers [(+)-catechin and (?)-epicatechin)]. Regarding proanthocyanidin oligomers, five procyanidin dimers (B1, B2, B3, B4, and B5) and one trimer (T2) are reported for the first time in P. niruri, as well as two propelargonidin dimers in S. reticulata. Additionally, P. niruri showed the highest antioxidant DPPH and ORAC values (IC50 of 6.4 μg/mL and 6.5 mmol TE/g respectively), followed by S. reticulata (IC50 of 72.9 μg/mL and 2.68 mmol TE/g respectively) and P. alliaceae extract (IC50 >1000 μg/mL and 1.32 mmol TE/g respectively). Finally, cytotoxicity and selectivity on gastric AGS and colon SW20 adenocarcinoma cell lines were evaluated and the best values were also found for P. niruri (SI = 2.8), followed by S. reticulata (SI = 2.5). Therefore, these results suggest that extracts containing higher proanthocyanidin content also show higher bioactivities. Significant positive correlation was found between TPC and ORAC (R2 = 0.996) as well as between phenolic content as measured by UPLC-DAD and ORAC (R2 = 0.990). These findings show evidence for the first time of the diversity of phenolic acids in P. alliaceae and S. reticulata, and the presence of proanthocyanidins as minor components in latter species. Of particular relevance is the occurrence of proanthocyanidin oligomers in phenolic extracts from P. niruri and their potential bioactivity
Academic literacy program implementation in an Ecuadorian University: a multinomial logit approach
Joel Alej,María Isabel Alvarado Sánchez,Silvia Mariela Méndez Prado,ro Rosado Anastacio
- , 2019, DOI: https://doi.org/10.1080/02673843.2018.1544084
Abstract: ABSTRACT The ‘FCSHlee’ program proposed by ESPOL Polytechnic University’s School of Social and Humanistic Sciences (FCSH, acronym in Spanish), consisted in the implementation of a reading requirement of culturally enriching book by subject. This study shares the experience of an academic literacy initiative in the Ecuadorian university, ESPOL’s case. The program aimed to enhance undergraduate students’ cultural level as well as oral and written communication skills. We develop a significant sample (N = 387) of the students that were part of the program and collected the data through surveys after its completion. The study describes details of the program, its scope, implementation, statistics, and conditions. Among the careers studied at the FCSH, Economics was the one that had a bigger margin of appreciation towards the program, which is a factor that could indicate that these groups of students possess reading orientation given the analytical characteristics of their study program
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